We report on sensitivity, reproducibility, concordance, DNA mixtures and carryover for EXT sample cartridges pre-loaded with GlobalFiler ® Express and AmpFLSTR ® NGM SElect™ Express, (Thermo Fisher Scientific, Waltham, MA) STR chemistries. Once the sample is loaded in the RapidHIT EXT sample cartridge, a DNA profile is produced after amplification, detection and automated data analysis. RapidHIT EXT sample cartridge allows a user to generate a forensic DNA profile from less than 250 pg of extracted and quantified DNA in less than 90 min with less than one-minute hands-on time. A new generation of the RapidHIT sample cartridge for RapidHIT ID has been designed and tested which allows the loading of extracted and quantified DNA. RapidHIT ® 200 and RapidHIT ID are rapid DNA systems developed by IntegenX (Pleasanton, CA) and validated for the use of buccal swabs. Rapid DNA Analysis is a hands-free "swab in - profile out" process which consists of automated DNA extraction, amplification, separation, detection, and allele calling without human intervention. However, PCR amplification and electrophoretic analysis remain largely manual. Routine DNA extraction and quantitation is readily available. Simplification and integration of the routine steps would dramatically improve turn-around time and reduce the risk of operator contamination. Senate sponsor Senator Orrin Hatch (R-UT) and lead co-sponsor Senator Dianne Feinstein (D-CA) as well as House sponsor Congressman James Sensenbrenner (R-WI) and lead co-sponsor Congressman Eric Swalwell (D-CA), along with the other 12 Senate and 24 House co-sponsors for their support. Crime scene DNA sample processing involves both complex and routine processing steps.
The turn-around time of urgent crime scene DNA samples is often far longer than desired by law enforcement.
0 kommentar(er)
